Mordak R, Dejneka G.J., Twardoń J., Dzimira S., Stefaniak T., Mrowiec J., Dzięcioł M.
Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland
Introduction: Mastitis is the one of the most common diseases in dairy cattle. The subclinical form of mastitis is described as the one of the most money consuming problem in the dairy production, because except the costs of necessary treatment, it causes losses resulting from reduced milk production. The new models and methods of clinical and subclinical mastitis treatment are still being developed, but the use of antibiotics is currently most popular. The restrictions in the use of antibiotics in the food production forces to look for the new methods of mastitis treatment. In the presented study we evaluated the usefulness of the homogenate of the antlerogenic stem cells (MIC -1) in the therapy of the subclinical mastitis in dairy cows during lactation.
Material and Methods: In the experiment 10 Holstein Friesians cows aged of 4-6 years has been used. All animals were kept in the same conditions and during the whole experiment had the same feeding protocol. Clinical examination of the general health status were performed and did not reveled any abnormalities. Clinical and laboratory test of the udder and milk discovered in all cows, the presence of subclinical mastitis, determined by elevated level of somatic cells in milk, as well as presence of bacterial microflora. There were no signs of clinical mastitis in none of the examined animals. During the first part of the experiment all animals received for the three continuous days 5 ml of homogenate of the antlerogenic stem cells (MIC -1) (concentration of 2mln/ml), administrated into the each quarter of mammary gland through the teat canal. From the first day of homogenate administration and for the next seven days detailed analysis of the status of animal and especially mammary gland was monitored. For this purpose throughout the duration of the experiment, every day in addition to the routine clinical examination, qualitative and quantitative analysis of the mammary secretion (milk cytology) was performed. Moreover at the beginning and at the end of trials the evaluation of the level of acute-phase protein (APP) in milk (serum amyloid A /SAA/) was performed. Also the bacteriological examination (with the antibiogram) of the mammary secretion was performed: at the beginning of trial, two and five days after the last homogenate infusion.
In the second part of experiment cows with diagnosed subclinical mastitis received traditional intramammary treatment with the use of antibiotic for the three continuous days (according the results of antibiogram) the same as described above diagnostic procedures were applied to evaluate the results of treatment.
Results: The evaluation of the behavior of the examined parameters did not revealed the decrease of the SCC (somatic cells count) in milk after homogenate infusion. The moderate increase in this parameter was even noted. Also the increased concentration of APP was initially observed in milk. The rate of the decline of this parameter in individual animals, and even between different quarters showed significant differences and was significantly slower (if happened) comparing to the traditional treatment. There was no influence of the homogenate infusion on the bacterial flora isolated from the milk samples. In contrast the traditional treatment with the use of selected antibiotics proved to be highly effective, reducing the presence of bacteria almost in all cases and reducing the elevated level of APP in milk samples. In most cases also SCC was significantly reduced.
Conclusion: Obtained results did not confirmed the positive effect of stem cells homogenate for the subclinical mastitis treatment in cows. Taking into account the specificity of mechanism of action of the stem cell therapy, the further studies are indicated to determine whether this product could be somehow useful in the regeneration of the teat injuries in cows.
 Characteristics of antlerogenic stem cells and their potential application. Cegielski et al. Tissue Engineering. Edited by Daniel Eberli MD PhD ISBN 978-953-307-079-7, 2010.
 Therapeutic effects of antimicrobial treatment during lactation of recently acquired bovine subclinical mastitis: two linked randomized field trials. Van den Borne BH, van Schaik G, Lam TJ, Nielen M. J Dairy Sci. 2010 Jan; 93(1):218-33. Doi: 10.3168/jds.2009-256.
"Project nr: UOD-DEM-1-351/001 financed by National Centre for Research and Development and by European Regional Development Fund under the Operational Programme Innovative Economy"