Mrowiec J., Mordak R., Niżański W., Dzięcioł M., Dejneka G. J., Nowak M. and Iwaszkiewicz M.
Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland
Objectives: Involution of the uterus in dairy cows is a rapid process ranged 45-50 days after parturition. Regeneration of endometrium is one of the crucial factor connected with future fertility of animals. Therapy used stem cell is a very important part of the regenerative treatment in human as well as in veterinary medicine. The aim of this study was to evaluate influence of the homogenate of the antlerogenic stem cells (MIC-1) on the uterine involution and fertility rates in dairy cows.
Material and Methods: The study was performed on 14 dairy Holstein-Friesian and 1 Polish Red-White cows, housed in the same free stall barn, fed with TMR. The average milk yield was 6,800 kg per cow per year. Animals were included to experiment 3-4 days after parturition if they were clinically health and delivery was physiological. Cows were divided into two groups: tested (T) and control (C) (n=7 and n= 8 respectively). On the first day of study all cows were sampled for cytological and microbiological examination as well as blood for morphological and biochemical evaluation were collected. Animals in group T received 60 ml of the homogenate of the antlerogenic stem cells (MIC-1), in C group 60 ml of placebo (aqua pro injectione) respectively. Animals were observed for next 40-45 days and every sings of illness or heat were recorded. After 45-50 days post partum the same samples as on the first day of study were collected. Additionally biopsy of endometrium was performed. Animals were inseminated when showed first sing of oestrus after samples collection. Pregnancy detection was performed between 28-35 after insemination with ultrasound scanner.
Results: Animals in both groups didn't show any sings of clinical illness or disease of genital track during the study. Morphological involution of the uterus and restart of ovarian function were found in all cows during second sampling day. Morphological and biochemical parameters of blood were maintained within normal limits. Any increase of infection rate was not observed in both groups. The degree of endometrium irritation was performed according to ISO 10993-10 norm (scale from 0 to 16 points). The degree of irritation ranged from 2 to 4 and from 0 to 6 in group T and C respectively. In group T 6 animals showed oestrus sings and 3 of them got pregnant. In group C 3 cows expressed oestrus behavior, 1 got pregnant.
Conclusion: Absence of clinical signs, changes in blood, uterine microflora and low level of endometrium irritation after intrauterine infusion of the homogenate of the antlerogenic stem cells (MIC-1) showed that this product is save. Differences in fertility rates between both groups indicated that it could have positive influence on uterine involution, but further studies are needed.
"Project nr: UOD-DEM-1-351/001 financed by National Centre for Research a Development and by European Regional Development Fund under the Operational Programme Innovative Economy".