Ahmed S. Al Mahruqi, Yousuf M. Al Suleimani and Gada Al-Suleimi
Department of Pharmacology and Clinical Pharmacy, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman
Noladin ether (NE) has recently been reported as an endocannabinoid, with selectivity for the cannabinoid CB1 receptor. In this study, we aimed at exploring the effect of NE on the contractility of small resistance arteries and the possible mechanisms involved in the effect. Second order branches of the superior mesenteric artery from rats were mounted in a wire myograph. NE (1-10 µM) caused concentration- and endothelium-dependent vasorelaxation. Pre-incubation with capsaicine (10 µM), ODQ (10 µM) and indomethacin (10 µM) significantly (P < 0.05) reduced vasorelaxation to NE, while the compound CID16020046 (2 µM) had no effect. In conclusion, NE is an endothelium-dependent vasorelaxant. The mechanism of vasorelaxation involves activation of TRPV1 channels, cGMP and cyclooxygenase.