Alireza Komaki, Masoumeh Asadbegi, Iraj Salehi, Parichehreh Yaghmaei, Azadeh Ebrahim-Habib, Siamak Shahidi and Abdolrahman Sarihi
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Materials and Methods: In this study 40 adult male Wistar rats were randomly assigned to five groups (n = 8 rats/group): Group 1 (Control; consumed an ordinary diet), Group 2 (vehicle group or HFD + phosphate-buffered saline [PBS]), Group 3: (HFD + Aβ), group 4: (HFD + Aβ + Thy) and group 5: (HFD + Aβ + Oil). Following recovery of AD induction and treatment period, rats were anesthetized with intraperitoneal injection of Urethane. After stereotaxic surgery, field potential was recorded after implanting the recording and stimulating electrodes in the dentate gyrus (DG) and perforant pathway (PP) respectively. Long-term potentiation (LTP) was induced in the hippocampal area by high-frequency stimulation of the PP. After that, population spikes (PS) amplitude as well as slope of excitatory post synaptic potentials (EPSP) were measured in DG.
Results: The HFD group receiving Aβ showed a significant decrease in EPSP slope and PS amplitude as compared to the control group (P≤ 0.05). On the contrary, HFD rats receiving Aβ and Thy exhibited lesser decrease in the EPSP slope and PS amplitude (P≤ 0.01).
Conclusion: Thy decreased the Aβ induced effect in the LTP at high risk rats with HFD administration. Therefore, we suggest that Thy consumption has a therapeutic effect against high risk Aβ impaired group that fed with HFD.
Keywords: Alzheimer''s disease; Aβ-induced; High-fat diet; Long-term potentiation; Thymol.