Sefeera A.A., Junise V., T.N.K. Suriya Prakash and Hafsa P.V.
Department of Pharmaceutics, Al Shifa College of Pharmacy, Perinthalmanna, Kerala, India
Studies were undertaken on the development and characterization of Chitosan Microsphere loaded with Rifampicin, with a view to develop extended release formulation. In this preparation, polymers (chitosan and sodium alginates) were used with a fixed concentration of Rifampicin [(1:1:1), (1:1:2), (1:1:3), (1:1:4), (1:2:1), (1:3:1), (1:4:1), and (1:2:2)]. Microspheres were prepared for all these concentration by ionotropic gelation/ external gelation technique. All formulations were showed good flow properties. Interaction studies like UV and IR spectra show that there were no interaction between drug and carrier used. The principle involved was the cation-induced gelation of alginate, for the simultaneous encapsulation of rifampicin. Ionotropic gelation was applied to prepare microspheres using combinations of chitosan and Ca2+ as cationic components and alginate as anion. The prepared Rifampicin microspheres were shown red color with elegant appearance, before drying and on drying it appeared as reddish brown color.
SEM and particle size analysis showed that the size of the particles were in micrometer range and all formulation showed average particle size below 10μm. And shows good % yield with good % drug content (75%-96%), and % encapsulation efficiency. Formulation 3, (RM-3) showed maximum entrapment efficiency and with maximum swelling capacity in both PH range, report showed that, as increasing the concentration of chitosan the swelling index also increased.
Results of dissolution studies shows that formulation 3 (RM-3) gives good release profile compared to other formulations. And pharmacokinetic evaluation shows all the formulation shows zero-order kinetics with Non-fickian mechanism of release and stability studies shows that the sample is stable for more than one year (At room temperature and on Refrigeration).