Masoud H. Golakani, Mohammad G. Mohammad, Hui Li, Manvendra Saxena, Samuel N. Breit, Marc Ruitenberg and David A. Brown
Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Sharjah, Sharjah, UAE
The dynamics of inflammatory responses post spinal cord injury (SCI) is largely unknown. In this study we aimed to investigate various immune cells in and around the injury site and their relationship with locomotor functional recovery at various time points post SCI.
Severe contusive SCI was induced and locomotor function was assessed at 7, 14, 21, and 28 days post injury (dpi). The entire spinal cord was removed, immune cells were isolated, characterized, and localized using FACS, immunohistology and immunofluorescence. Various immune cells/subsets were analyzed for correlation with behavioral assessment at various time points post injury.
Macrophages and CD11c and B220+ dendritic cells had both beneficial and detrimental effects depending on the time post SCI. While macrophages had adverse effects on locomotion recovery in early stages of 7-14 dpi, they significantly contributed to improved recovery at late stages of 21-28 dpi. CD11c&B220+ dendritic cells on the other hand were advantageous in early stages and detrimental on more chronic stage of SCI.
These findings highlight the importance of understanding the dynamics of inflammatory response and its relation to functional recovery, this may be therapeutically manipulated.