Argi Anuradha, Veerathu Lakshmi Kalpana, Kirmani Natukula, Peela Jagannadha Rao and Janipalli Sarat Babu
Faculty of Medicine, Quest International University Perak, Ipoh, Malaysia
Aim: To analyse the association between polymorphisms of GST genes and the risk for oral cancer.
Materials and Methods:-A case-control study using molecular epidemiological method was conducted. Various polymorphism of GST gene from DNA samples of 105 cases and 110 controls were detected by PCR method.
Results: Since the mean age of present study patients was 55 years and highest HNC cases were observed in age group of 21 to 30 and 61 t0 70 years, the age proved to be an uncontrollable variable with the increase in age particularly between 40 to 60 years.Null genotype in GSTM1 gene with smoking cigarette or Bidi and wild type genotype with all tobacco habits in GST T1 gene was found significant.86.1% were smokers and 24.1%were non-smokers. The incidence of HNC in smokers is approximately two fold compared with non-smokers.GSTT1 positive genotype shows significant results in relation to tobacco chewers and tobacco/bidi smokers but insignificant with null genotype. Non smokers were also found to be significant with null genotype. The null genotype of GSTT1 is in significant with the smokers.
Conclusion:-Null genotype in GSTM1 gene with smoking cigarette or Bidi and wild type genotype with all tobacco habits in GST T1 gene was found significant. Risk increases when the metabolic enzymes as products of these genes seem to interact with tobacco carcinogens, the mechanism of which is still not understood.
Keywords: Polymorphisms, Genotype, Carcinogenesis.