Ahmed T. El-Serafi, Yasmin Lozansson and Divyasree Sandeep
College of Medicine, University of Sharjah, UAE
Burn victims and mastectomy patients have high psychological burden and low quality of life, especially that the current management plans are not physiological and associated with complications and co-morbidities. Mesenchymal stem cells have been studied for their ability to differentiate into adipocytes with controversial results. Epigenetic modifiers have been shown to enhance the stem cell differentiation by rendering the genome more responsive to the culturing condition. In this study, we investigated the effect of the DNA methylation inhibitor 5-Aza-dC and the histone deacetylse inhibitor Suberoylanilide hydroxamic acid (SAHA) as an additive to the classical adipogenic differentiation protocol in MG63 cell line, which has the characteristics of mesenchymal stem cells. Fat accumulation, as evident by oil red staining, was enhanced in SAHA pretreated cells while decreased with 5-Aza-dC in comparison to the vehicle or control groups. The same pattern has been shown with gene expression including the peroxisome proliferator-activated receptor gamma, adiponectin receptor type I and II and glucose transporter type 4. The secretion of monocyte chemotactic protein-1, an adipogenic inducer, reached up to 165% in the culture supernatant with SAHA and less than 22% with 5-Aza-dC in comparison to the control. This study does not only highlight the potential role for SAHA in fat regeneration but also open the door to investigate the potential role of 5-Aza-dC as anti-obesity agent.