Gerardo F. Goya and M.Ricardo Ibarra
Nanoscience Institute of Aragon (INA), University of Zaragoza, Pedro Cerbuna 12, 50009- Zaragoza, Spain
We aim to investigate the effects of alternating magnetic fields (AMF) on the death rate of magnetic nanoparticles uploaded dendritic cells (DCs). Specific parameters as the time of application; the magnetic field amplitude and the concentration of magnetic nanoparticles (MNP) used as heating agents have been investigated in order to assess the best conditions for a controlled field-induced cell death.
The magnetic hyperthermia experiments were carried on monocyte-derived DCs at f = 260 kHz and field amplitudes H up to 12.7 kA/m. Application times of 5, 10 and 15 min were tested for several concentration of co-incubated MNPs (from 1 to 50 µg MNP/ml medium). Cell viability was measured by Trypan blue, MTT and flow cytometry using annexin-propidium iodide markers.
We were able to control the DCs viability by choosing a) field amplitude; b) application time; and c) the concentration of co-incubated MNPs. Large decrease of cell viability induced by AMF was observed without heating of the cell culture . In such a case AMF-triggered mechanisms could be the origin of the observed cell death, yielding a different cell death pathway.
We propose that the cell death of magnetically loaded DCs constitutes an appealing strategy to be used as a non-invasive and selective hyperthermia method. This can be controlled by adequate tuning of the physical AMF parameters and/or MNPs concentration. Power absorption by the MNPs might locally trigger metabolic processes yielding fast and irreversible cell damage . We have addressed the effects of radio frequency fields and determined the strong effect on MNP loaded eukaryotic cells .
Keywords: Magnetic nanoparticles, deep cancer treatment, magnetic hyperthermia.
 I Marcos-Campos, L Asin, T E Torres, C Marquina, A Tres, M R Ibarra and G F Goya, Nanotechnology 22 (2011) 205101,doi.org/10.1088/0957-4484/22/20/205101
 L. Asín, G.F. Goya, A. Tres and M.R. Ibarra, Cell Death and Disease (2013) 4, e596; doi:10.1038/cddis.2013.121
 G.F. Goya, L. Asín and M.R. Ibarra, Int J Hyperthermia, (2013) 29(8) 810-8. doi: 10.3109/02656736.2013.83864