Invited
Speaker
The Roles of High-Brilliant Synchrotron Radiation in Macromolecular Crystallography
Masaki Yamamoto
Japan
InIn the third generation synchrotron
radiation, macromolecular crystallography is one of the major subjects
in the past decade. At SPring-8, 8 beamlines are dedicated for macromolecular
crystallography. We have been constructing and developing the beamlines
with the view of two objects. One object is improvements of the applicable
crystals size and the data quality, and the other is the high throughput
data collection for Structural Genomics research.
BL41XU is an undulator beamline with the high brilliance of 4.8 ×
1014 photons/sec/mm2. The K/B mirror system was installed for micro
crystals (~ 20 µm) using a small beam. The beam size at sample
position is controllable from 20 × 20 to 70 × 100 µm2.
SAD measurements of Se-methionine samples with the crystal size from
15 µm were performed, and we solved the initial phase at the
resolution of 2.7 Å. A new undulator beamline dedicated for
protein micro-crystallography, named RIKEN Targeted Proteins Beamline
(BL32XU), is under commissioning, which will start operation from
April 2010. A small sized and highly brilliant X-ray beam with size
of 1 micrometer at BL32XU will be providing high S/N data.
BL26B1&B2 have been constructed for Structural Genomics research1).
The beamline operation is automated cooperating with the sample changer
robot named SPACE2), the operation software BSS3) and beamline management
database D-Cha. Combination of BSS, SPACE and D-Cha also enables Mail-in
data collection, and the Web interface of D-Cha allows users to deposit
measurement condition or to observe recorded images from distant user
laboratory.
We will present the present status and the future prospects of macromolecular
crystallography beamlines at SPring-8. BL32XU project was supported
by Targeted Proteins Research Program 4) from the Ministry of Education,
Science and Culture (MEXT) of Japan.
1) Ueno, G. et al. J. Struct. Funct. Genomics, (2006), 7,
15-22.
2) Ueno, G. et al. J. Appl. Cryst. (2004), 37, 867-873.
3) Ueno, G. et al. J. Synchrotron Rad. (2005), 12,
380-384.
4) http://www.tanpaku.org/e_index.html
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