Poster Presenter
Low Dose Cinnamaldehyde Increase The Survival Time In Different
Stress Conditions In Zebrafish (Danio Rerio) Model System
Vradi L., Buza E., Csenki Zs., Jeney Zs., Mller
T.,Urbnyi B., Mzes M
Cinnamaldehyde is a well-known natural anaesthetic in fishes
however its effect in different stress conditions, even at very low
concentration, did not prove earlier. The purpose of present study
was to investigate the effect of cinnamaldehyde on survival time of
zebrafish, as model animal, in different periods of stress conditions
- cold stress and social ("mass") stress followed by relaxation
and hypoxia.
AB line zebrafish population at the same age and size was used for
the investigations. Partially purified cinnamaldehyde solution (Aetherolium
cinnamomi, 80% v/v cinnamaldehyde content) was dissolved in the aquarium
water at the same concentration 0.05 ml L-1 in all trials, based on
the results of preliminary experiments.
Cold stress was induced at 14 oC (initial temperature 24oC),
and social ("mass") stress with the increase of stocking
density per volume of water first for short (5 minutes) and longer
(10 minutes) periods followed by different length (10 and 60 minutes
and 22 hours) relaxation period at 24 oC or normal stocking
density. After that relaxation period the survival time was determined
at hypoxic condition.
It was found that cinnamaldehyde significantly increased the survival
time in hypoxic condition after cold stress as compared to untreated
controls using 10 minutes relaxation period but not longer ones (60
minutes or 22 hours). However the mean survival time was significantly
shorter in cinnamaldehyde treated fishes kept longer (10 minutes)
period in cold stress as compared to the shorter (5 minutes) ones.
In case of social stress cinnamaldehyde also increased significantly
the survival time in hypoxic condition after 10 minutes relaxation
period as compared to the untreated controls irrespective of the length
of the first stress period. Longer relaxation periods (60 minutes
or 22 hours) also did not have measurable effect on the survival time.
The results suggested that cinnamaldehyde, even at low concentration,
has significant effect on the survival time in hypoxic co ndition
in zebrafish after shorter or longer term cold or social stress followed
by short (10 minutes) relaxation period. The route of absorption of
cinnamaldehyde from water to the fishes, and the exact mode of action
is not known, yet. The results suggested that cinnamaldehyde affect
mainly the first phase, catecholaminaemia, of stress, but it has less
effect on the later, hypothalamo-pituitary-adreno-cortical, phases.
However further research requires for proven of that hypothesis.
The experimental protocol was approved by the local State Veterinary
Office according to Hungarian Animal Welfare Law (22.1/518/003/2008)
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