Poster Presenter

Photodynamic Treatment on Cancer Cell Lines
Hana Kolarova, Katerina Tomankova, Petr Kolar, Miroslav Strnad
Czech Republic

Photodynamic therapy (PDT) is a treatment modality of tumors. The cytotoxicity results from a combined effect of sensitizer, oxygen and light. During these processes reactive oxygen species (ROS) are produced that are involved among major agents triggering cell death by apoptosis or necrosis mechanism. The resulting damage to organelles within malignant cells leads to tumor ablation. The promising generation of sensitizers - phthalocyanines were used to induce the photodamage and apoptosis. The ability of PDT to produce a rapid apoptotic response may be an important element of successful photodynamic therapy. In this study, the antitumor effect of ClAlPcS2 induced by PDT was evaluated on G361 melanoma cells by fluorescence microscopy, atomic force microscopy (AFM) and quantitative fluorescence measurement of ROS production. Our results show ROS production in G361 melanoma cells is dependent on the sensitizer concentration of ClAlPcS2, and irradiation. This work was supported the grant projects MSM 6198959216 from the Ministry of Education, Youth and Sports of the Czech Republic IGA NS 9648-4.