Poster Presenter

Effect of Triamcinolon in Liposomes on Inducible Nitric Oxide Syntetase Expression in Oral Wound Healing In Rats
Petelin Milan and Vladimira Erjavec
Slovenia


The aim of our study was to determine, whether we can successfully treat oral mucosa wounds in rats with the active ingredient triamcinolone acetonide (TA), included into multilayered liposomes made from hydrogenated soy lecithin of different sizes and in several times lower concentration than recommended. A round wound of 3 mm in diameter was made on oral mucosa of adult female Wistar rats. This wound was topically treated 6 days with liposomes without TA, and liposomes with 0.01% and 0.05% concentration of TA. The wounds of the animals in control group were not treated. Polymethyl metacrylate (PMM) was used as an ointment for mixing in liposomes. The area of inflammatory infiltrate under the wound was the smallest in the group, treated topically with liposomes without TA, the iNOS was also expressed the least in this group, and it was more expressed in untreated control group. iNOS was the most expressed in the groups, receiving TA. On the day 2 of the experiment angiogenesis was expressed the most in the group receiving TA. We assume excessive expression of iNOS enzyme with large quantities of produced NO to have a toxic effect and may delay oral mucosa wound healing in rats.