Poster Presenter

Fresh Bee Pollen Characterized by Nutritional, Antioxidant and Volatile Component
Patricia vit, Gina Meccia, Luis Rojas, Alfredo Usubillaga, Antonio Rodríguez- Malaver, and Monika Barth
Brasil

Pseudomonas aeruginosa is an important opportunistic pathogen causing widespread infections and increasing resistance to antibiotics makes its treatment difficult. The purpose of this study was to evaluate antimicrobial characteristics of essential oils from Matricaria chamomilla, Artemisia persica, Zataria multiflora, Myrtus communis, Ruta graveolens, Eucalyptus camaldulensis and Ferula gummosa on Pseudomonas aeruginosa (ATCC 27853). The selected essential oils were screened against Pseudomonas aeruginosa using disc diffusion method. The minimal inhibitory and bactericidal concentrations (MIC and MBC) of the active essential oils were tested using macro dilution method at concentrations ranging from 0.125 to 256μg/ml. The most potent oils i.e. Zataria multiflora, Myrtus communis and Eucalyptus camaldulensis were analyzed by GC/MS. In this study, three of the seven essential oils (Zataria multiflora, Myrtus communis and Eucalyptus camaldulensis) were significantly active against Pseudomonas aeruginosa exhibiting MIC/MBC of 64/128, 64/64 and 64/128 respectively. Gas chromatography mass spectrometry (GC/MS) analysis led to identification of 32, 21, and 22 components in Myrtus communis, Eucalyptus camaldulensis and Zataria multiflora oils respectively. With a view to antibacterial activity of some oils against the tested bacterium, their safe antibacterial potentials can therefore be exploited as alternative agents in combating infections of Pseudomonas aeruginosa origin.