Poster Presenter

Monensin, Toltrazuril and the Mix of Them at 24h and 48 h on Oocysts of Cryptosporidium parvum in Hank's Balance Salt Solution
Maryam Fathi, Javid Sadraei, Fathemeh Ghaffarifar

Cryptosporidium parvum is a common case of diarrhea in both animals and humans worldwide. There is no effective specific chemotherapeutic intervention for cure, recently. The effects of various preparations of two anticoccidial drugs (Monensin and Toltrazuril) and the mix of them on oocysts of Cryptosporidium parvum at 24h and 48h in Hank's balance salt solution (HBSS) were studied. Oocysts of C. parvum were isolated from the fecal samples of calves and stored in HBSS in 4°C until use. Different concentrations of Monensin and Toltrazuril and the mix of them (0, 0.1, 0.5, 1, 10, 20, 60 and 100 made by HBSS were exposed for 24h and 48h on the oocysts of C. parvum. The effects of the drugs on the purified C. parvum oocysts were evaluated by counting the complete oocysts with four sporozoites after 24h and 48h incubation at 37°C.The results showed a significant decrease in the oocysts number related to the increase in the concentration and exposure time of the drugs. The mix of two drugs had the highest efficacy on the C. parvum oocysts than each of drugs alone at 24h and 48h (P <0.001). In addition, Monensin in contrast to Toltrazuril at the same concentration showed to be more effective on C. parvum oocysts at 24h and 48h (P < 0.001).