Poster Presenter

Design, Synthesis And Evaluation Of New bis-DNA Intercalators As Possible Anti-Cancer Agents
Aruna Devi Mahesh and Malla Reddy Vanga
India

Nine new bis (4-substitued benzoyloxy ) alkanes were synthesized by reacting the substituted benzoylchloride with different alkanediols. All the new compounds were characterized by infrared (IR), 1H nuclear magnetic resonance (NMR) and mass spectroscopy. The compounds were evaluated for DNA binding studies. The DNA binding studies reveal the symmetrical bis-DNA intercalators possess higher DNA binding capacity. Anticancer activity of these compounds were evaluated on cell lines by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) method. The MTT assay and the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay are laboratory tests and standard colorimetric assays (an assay which measures changes in color) for measuring the activity of enzymes that reduce MTT or MTS + phenazine methosulfate (PMS) to formazan, giving a purple color. It was found that the compounds possessing electron withdrawing groups at 4-substituted phenyl ring exhibited anti cancer activity.