Poster Presenter

The Effect of B12 Vitamin Solid Lipid Nanoparticle 5RP7 Cell Line
M. Kutlu, L. Genç, G. Güney, A.şcan
Turkey

Vitamin B12 is a water soluble vitamin with a key role in the normal functioning of the brain and nervous system. Vitamin B12 is normally involved in the metabolism of every cell of the body, especially affecting the DNA synthesis and regulation.Vitamin B12 have a number of biological roles that potentially make them important in cancer. <

Solid lipid nanoparticles (SLN) have been introduced to the literature as a novel carrier system pharmaceutical drugs. Compared to other delivery systems such as liposomes and polymeric nanoparticles, SLN possess various advantages. Examples are production without organic solvents, long time physical stability and the possibility of protection of chemically labile actives inside the particles. SLNs were prepared by homogenization method. Compritol® was used as the lipid. For the characterization of the formulations obtained, particle size distribution, Zeta potential measurement, thermal behavior (DSC), active agent content and pH were investigated.

We postulated that, B12 solid lipid nanoparticle interaction with rat embryo fibroblast cells, 5RP7. We examined the cytotoxic effects of SLN B12, again on 5RP7 cells and determined cellular and ultrastructural of 5RP7 cell by using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide), TEM.