Poster Presenter

ESTIMATION OF TRANSPORT CAPACITY OF HUMAN SERUM PROTEINS AT LIVER PATHOLOGIES WITH USE OF TRITUM LABELED PHARMACOLOGICAL PREPARATIONS
Gulnora Talipovna DJURAEVA, A.A.Kim, B.V. Shukurov, I.R.Mavlyanov and Dadakhanov J.A.
Uzbekistan

The radiochemical micromethod on the basis of tritium labeled pharmacological preparations furosemide and drotaverine (no-spa) has been used for an estimation of the binding capacity of human serum proteins at various liver pathologies. 20 microliters of serum samples were incubated in vitro with tritium labeled drotaverine and furosemide. After incubation serum proteins were fractionated by chromatography and tritium radioactivity bound with fraction of serum proteins was measured.

Research was carried out on pediatric patients in the age of 3 to 14 years with acute and chronic hepatitis A and B. The dynamics of changes of binding capacity of serum proteins during standard therapy of hepatitis A and B. It was found that during convalescence at application of standard therapy the binding capacity of serum proteins comes nearer to values of control group. Thus dynamics of changes of binding capacity of serum proteins of patients with hepatitis B differed from dynamics of patients with hepatitis A.

Obtained results in the whole indicate the reducing of binding capacity of serum proteins at virus hepatitis that allows to determine the optimal strategy of a pharmacological load on patient organism and thus to optimize the treatment of patients.

Carried out research has shown good efficiency and accuracy of application of a radiochemical micromethod on the basis of tritium labeled pharmacological preparations for estimation of capacity of transport system of blood proteins at liver pathologies.