Poster Presenter

Proteomics Analysis of Cellular Proteins of Human Lung Cells in Response to Avian H5N1 Virus Infection
Andrea Fuleova, Ludovit Skultety, Stephan Ludwig, Rudolf Toman
Slovakia

From December 2003, an unprecedented H5N1 epizootic in poultry and migrating wild birds has spread across Asia and into Europe, the Middle East, and Africa. Humans in close contact with sick poultry and on rare occasion with other infected humans, have become infected. We focused on proteomics analysis of the cellular responses in A549 human lung epithelial cells after 24 h post-infection with H5N1 virus. The analysis allowed clustering proteins involved in particular cellular functions COG: C, energy production and conversion (2 proteins), D, cell division and chromosome partitioning (2), E, amino acid transport and metabolism (2), G, carbohydrate transport and metabolism (1) H, coenzyme metabolism (1), J, translation, ribosomal structure and biogenesis (4), K, transcription (2), L, DNA replication, recombination and repair (1) O, post translational modification, protein turnover, chaperones (11), and R, general function prediction only (1). Thus, a set of differentially expressed cellular proteins involved mainly in post translational modifications, protein turnover and translation, ribosomal structure and biogenesis were established. The data presented will help to elucidate basic processes that take place during infection of H5N1 influenza virus in human epithelial lung cells and will enhance search for protein candidates for viral pathogenesis/adaptation studies.