Poster Presenter
Enhancement Of The Cytostatic Therapeutic Potential By Flavonoids
Galova, E., Miadokova, E., Nadova, S., Sevcovicova, A., Kopaskova, M., Hudecova, A., Hasplova, K.
Slovakia

The high incidence of cancer and the need of characterization of new natural substances (e.g., flavonoids), which would have been involved in the cancer prevention and therapy, stimulated our study for their beneficial effects. Flavonoids may not serve only as drugs or templates for drugs directly, but in many cases lead to a better understanding of targets and pathways involved in the disease processes, as well as to enhancement of the therapeutic potential of chemotherapeutics as we revealed by their combinational treatment with cytarabine and cis-platinum. Because one tumoral inhibition mechanism happens via induction of apoptosis in cancer cells we focused on its analysis by electrophoresis, fluorescence microscopy and flow cytometry. A rational use of chemopreventive agents is based not only on assessment of their efficacy but also on their mechanisms of action understanding. Several mechanisms, such as antigenotoxic/antioxidant activity, cell proliferation inhibition and signal transduction modulation can be involved and therefore were elucidated in our research. Because many natural compounds occur as mixtures with a complex array of molecular interactions we decided to study their biological activities in plant extracts as well as their single constituents to provide important information for plant drug research.