Poster Presenter

Resveratrol Inhibits Streptozotocin-induced Diabetes by Preventing Pancreatic β-Cell Apoptosis through Inhibition of the Cleavage of Poly(ADP-ribose) Polymerase
Lee H.C., Ku C.R., Lee M-K., and Lee E.J. Seoul
USA

Objective:Resveratrol (3,5,4¢-trihydroxystilbene; RSV) is one kind of polyphenolic phytoalexin that has many effects on metabolic diseases. This study aimed to evaluate the protective effect of RSV pretreatment on β-cell.

Materials and Methods:Male Sprague Dawley rats weighing 200–230 g were divided into 4 groups: (1) RSV; (2) streptozotocin (STZ, 70 mg/kg, intraperitoneally); (3) STZ after 7 days pretreatment with RSV; and (4) STZ pretreated with nicotinamide. Fasting glucose concentration was measured and an intraperitoneal glucose tolerance test was performed 72 h after STZ injection to determine the diabetic condition. The pancreas was removed 3, 6, 36, and 48 h after STZ injection.

Results:STZ induced diabetes in all rats not given RSV pretreatment, whereas none of the RSV-pretreated rats developed diabetes. Pretreatment with RSV inhibited apoptosis and the activation of caspase-3 and poly(ADP-ribose) polymerase (PARP). However, expression of the total length PARP was not affected by pretreatment.

Conclusions:RSV protects β-cells from STZ by inhibiting the activation of PARP.