Poster Presenter
The In Vitro Effects Of
α-Solanine
On MDA-MB-231 Breast Cancer Cells Are Time- And Concentration-Dependent
Tan KH Benny, Woo CC, Hsu A
Singapore
There are several types of cell death,
such as apoptosis, apoptosis-like cell death, necrosis and autophagic
cell death. Necrosis provokes a stronger immune response than apoptosis;
this in turn attracts more immune cells to abnormal or tumour cells.
In this study, the type of cell death induced by α-solanine, previously
shown to have cytotoxic effect in MDA-MB-231 breast cancer cell
line, was investigated, using the Cell Death Detection ELISAPLUS
kit. Cells were incubated with varying concentrations of α-solanine
(5.0-50 μg/ml) for 4, 12 and 24 hours. The results showed
that α-solanine at the lower range of concentrations was not able
to induce significant cell death even after a period of 12 hours.
However, after 24 hours, there was significant evidence of cell
death, with necrosis being the dominant form from concentrations
above 10 μg/ml. Microscopy showed characteristics of apoptosis
at 12 and 24 hours. This preliminary study suggests that α-solanine
induced both apoptosis and necrosis in MDA-MB-231 cells. It may
be potentially useful in the treatment of breast cancer.
References
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2. Schwartz LM, SW Smith et al. Proc Natl Acad Sci USA 1993; 90:
980-4
3. www.roche-applied-science.com
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