Poster Presenter
Inhibitory Activity of Caffeoylquinic
Acids from Artemisia princeps on Diabetic Complications
Young Eun Jeon, Yeon Sil Lee, Se Jin Choi, Il-Jun
Kang and Soon Sung Lim
Republic of Korea
Caffeoylquinic acids–3, 4-O-dicaffeoylquinic acid
(1); 1,3,5-I-tricaffeoylquinic acid (2);
and 3,4,5-O-tricaffeoylqunic acid (3)––were
isolated from an acetone-soluble fraction of the aerial parts of
Artemisia princeps. Their structures were determined spectroscopically
using 1D- and 2D-nuclear magnetic resonance (NMR) studies, as well
as by comparing the NMR results with previously published structures.
All the isolates were subjected to in vitro bioassays to
evaluate their efficacy in inhibiting rat lens aldose reductase
(RLAR) activity and the formation of advanced glycation end products
(AGEs). We found 1,3,5-O-tricaffeoylquinic acid (2)
to be the most potent AGE inhibitor, and the concentration that
resulted in 50% inhibition (IC50) was 22.18 ±
1.46 μM, as compared to the aminoguanidine and chlorogenic
acid controls, which had IC50 values of 1,093.11 ±
10.95 μM and 117.63 ± 0.20 μM, respectively.
In the RLAR assay, the three caffeoylquinic acids were found to
have IC50 values in the range of 1.78–2.40 µM, demonstrating
a 5- to 10-fold greater efficacy in RLAR inhibition as compared
to the quercetin control, which had an IC50 value of
17.91 Mm.
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