Poster Presenter

Construction Of Eukaryotic Recombinant sTRAIL Expression Vector And Its Effects Of Anti -C6 -Glioma Activity In Vitro
Wusi Qiu, Chenchen Guo, Weiming Wang,Weiguo LIU
China

Objective: To construct eukaryotic recombinant sTRAIL expression vector and investigate its activity against C6 glioma cell line.

Methods: After construction of eukaryotic recombinant sTRAIL expression vector-PcDNA -sTRAIL by means of PCR and directed cloning, the C6 glioma cell line was transfected by cationic liposome and the proliferation and apoptosis of cells was detected by FCM.

Results: The construction of eukaryotic recombinant sTRAIL expression vector- PcDNA-sTRAIL confirmed a success by gene sequencing and western blot. After transfected by cationic liposome, 50 percents of C6 cells expressed positive protein. The apoptosis rate of C6 cell was significant higher in PcDNA-sTRAIL plus Lipofectin transfected group than that in three control groups (t =9.52, 7.20, 2.84 respectively, P <0.05). Conclutions: An eukaryotic recombinant sTRAIL expression vector was successfully constructed and its anti-C6-glioma activity was confirmed in vitro study.