Poster Presenter

Ileal Injury In Two Mice Models Of Microbial Sepsis: Probable Causes And Possible Protective
Hossam Ebaid and Rasha R. Ahmed
Egypt

Sepsis incidence and deaths are rising everyday despite the numerous researches and new therapeutic advance. Sepsis is associated with ileal injury. This study aims to determine the causes of ileal injury in two models of sepsis and the possible protective role of phytic acid. Male Swiss mice were assigned into the following: 1)- the control group, 2)- the bacteria group injected intraperitonealy with bacteria at a dose of 0.2 ml/ mice/week, 3)- the bacteria-treated group infected with bacteria and synergistically orally administered, three times/ week, phytic acid by gastric intubations at a dose of 40mg/kg b. wt., 4)- the endotoxin group (LPS) intraperitonealy injected with endotoxin once/ week at a dose of 20 mg/kg b. wt. and 5)- the endotoxin-treated group given LPS and synergistically treated with oral administration of phytic acid. Results showed an increased inflammatory and lymphocytic cells' influx associated with apoptotic index decrease. The inflammation was accompanied by hyper-mucus secretion, villar atrophy, necrosis and desquamation with each infection and was much severe in LPS. Most enterocytes of the infected mice lost their microvillar brush border and had destructed organelles. The morphometric studies recorded significant decrease in all examined measures (villar, enterocyte and microvillar heights, crypt depth and the goblet cells number) after four weeks of the onset of the experiment with both models. Phytic acid had the ability to attenuate ileal injury in the two models of sepsis after four weeks of its administration where its supplementation can greatly minimize the histopathological and cytological complications and morphometric alterations resulted from bacteria or its endotoxin. Its administration for four weeks was better for inducing its ameliorative effect via increasing mucus secretion, decreasing apoptotic index, attenuating the inflammatory and lymphocytic cells' count or increasing the renewal of the crypt cells and villar epithelial cells proliferation.