Poster Presenter

Studies on the Antiviral Mechanism of Virus-Suppressing Factor, VSF and its Recombinant Antibody
Kwang Soo Lee, Seong Ji Woo, Hye Jin Jang, Cho Hyei Ham and Sung Ho Jeon
Korea

VSF is the mouse immunoglobulin containing γ3 heavy chain and κ light chain expressed in B-cell hybridoma. In this study, we have constructed a single chain fragment variable region (scFv) from the VSF. VSF and its recombinant antibody scFv showed functional activity against viral infection. We here confirmed that VSF and scFv bound to the 60KDa cellular protein (p60) by immunoblot assay and immunofluorescence. Cellular phosphorylation events during viral infection are necessary for effective viral replication. We have examined the phosphorylation of mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinase (ERK1/2), p38 MAPK, and stress-activated protein kinase 1/c-Jun NH2- terminal kinase (SAPK/JNK) in EMC-D virus infected L929 cells. We found that the intracellular signaling through p60 induced by VSF, scFv and MAPKs inhibitor, U0126 suppressed the translation of EMC viral RNA. We conclude that VSF and scFv play a critical role in the replication of EMC-D virus, probably in the translation of viral RNA. These results are of interest not only in view of possible immunotherapy against EMC-D virus infection, but also more generally in emphasizing the great promise of these antiviral molecules in the context of targeted therapies.

Following are results of a study on the "Human Resource Development Center for Economic Region Leading Industry" Project, supported by the Ministry of Education, Science & Technology(MEST) and the National Research Foundation of Korea(NRF).