The 2nd International Conference on Drug Discovery & Therapy: Dubai, February 1 - 4, 2010

Poster Presenter

Thermosensitive chitosan-based hydrogel releasing Osteogenic Protein-1 for cartilage regeneration
Seung Soo You, Kyung Min Park, Yoon Ki Joung, Ki Dong Park
Republic of Korea

Chitosan-based hydrogel is a good candidate for cartilage regeneration, since it shares some characteristics with a variety of glucosaminoglycan (GAG) which is one of the major macromolecules found in articular cartilage. In this study, we designed thermosensitive chitosan-Pluronic (CP) hydrogel as a cell supported scaffold for cartilage regeneration. The CP copolymer was synthesized by grafting Pluronic onto chitosan using EDC/NHS chemistry. CP hydrogel showed the sol-gel transition around body temperature and the storage modulus (G') was over 104 kPa, showing potentials of injectable hydrogel for cartilage regeneration. CP hydrogel was rapidly formed by increasing temperature. In vitro growth factor release study was performed using osteogenic protein-1 (OP-1). OP-1 was released sustainedly from CP hydrogel for 15 days. In vitro cell culture was performed using bovine chondrocyte. The proliferation of bovine chondrocyte and amount of synthesized GAG were increased for 28 days. Therefore, the obtained results suggested that CP hydrogel has a potential for cartilage regeneration and it can be also useful as a new biomaterial for tissue engineering.

Acknowledgement: This work was supported by the grant from Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs, Republic of Korea (A091120).

















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