Poster Presenter
Thermosensitive chitosan-based hydrogel releasing Osteogenic
Protein-1 for cartilage regeneration
Seung Soo You, Kyung Min Park, Yoon Ki Joung,
Ki Dong Park
Republic of Korea
Chitosan-based hydrogel is a good candidate for cartilage regeneration,
since it shares some characteristics with a variety of glucosaminoglycan
(GAG) which is one of the major macromolecules found in articular
cartilage. In this study, we designed thermosensitive chitosan-Pluronic
(CP) hydrogel as a cell supported scaffold for cartilage regeneration.
The CP copolymer was synthesized by grafting Pluronic onto chitosan
using EDC/NHS chemistry. CP hydrogel showed the sol-gel transition
around body temperature and the storage modulus (G') was over 104
kPa, showing potentials of injectable hydrogel for cartilage regeneration.
CP hydrogel was rapidly formed by increasing temperature. In vitro
growth factor release study was performed using osteogenic protein-1
(OP-1). OP-1 was released sustainedly from CP hydrogel for 15 days.
In vitro cell culture was performed using bovine chondrocyte.
The proliferation of bovine chondrocyte and amount of synthesized
GAG were increased for 28 days. Therefore, the obtained results suggested
that CP hydrogel has a potential for cartilage regeneration and it
can be also useful as a new biomaterial for tissue engineering.
Acknowledgement: This work was supported by the grant
from Korea Health 21 R&D Project, Ministry of Health Welfare &
Family Affairs, Republic of Korea (A091120).
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