Poster Presenter

The Antibacterial and Cytotoxic Effect of Rumen Flora
Saeb Nazmi El-Sukhon
Jordan

Dehydrated crude whole cultures of 59 bacterial strains isolated from animal rumen contents were examined for their antibacterial activity, cidal effect on brine shrimp and cytotoxicity. Of those, 38 isolates revealed inhibitory effect on Micrococcus luteus ATCC 9341 and the Streptococcus faecalis ATCC 29212 with a zone diameter ranged from 7 to 20 mm. Nineteen strains were cidal to the hatched brined shrimp and their LD-50 values were recorded. Only 9 isolates showed both antibacterial and anti-shrimp effects. To determine the anticancer effect using the A-549 (lung carcinoma), MCF-7 (Breast cancer) and HT-29 (Colon adenocarcinoma) human cell lines, the protocol of National Cancer Institute, USA was applied on 11 strains. Relatively, higher effective concentrations compared with that of the control Adriamycin were recorded. The obtained results were encouraging and thoroughly discussed.