Poster Presenter

Association of Ddah2 Gene Polymorphism with Cardiovascular Disease in Egyptian Patients
M.Z.Gad, S.I. Hassanein, S.M. Abdel-Maksoud, G.M. Shaban, H.A. Elgabarty
Egypt

This study was undertaken to detect dimethylarginine dimethylaminohydrolase-2 (DDAH-2) (SNP2, - 449 C/G, rs805305) gene polymorphism among Egyptian patients, to correlate genotype profiles with plasma ADMA, SDMA, L-arginine, and C-reactive protein (hsCRP) levels, and to assess their association with risk of cardiovascular disease (CVD) in young susceptible individuals. Ninety four Egyptian male CVD patients (35-50 y) were recruited for the study from the National Heart Institute, Egypt. They were classified according to severity of coronary insufficiency based on coronary angiography. Age and sex-matched controls (n = 100) were included. Results revealed a trend of decreased GG and increased CC genotypes moving from the least to the most affected subjects with coronary insufficiency. AMI patients lack GG genotype, whereas 18% of controls had this genotype. On the other side, CC genotypes in AMI and in patients advised to do GABG operation were ≥ 2-folds higher than those of controls. No significant association between biochemical parameters and carriage of specific DDAH2 allele or genotype was detected. AMI Patients showed higher plasma levels of ADMA, SDMA, and hsCRP; and lower plasma L-arginine and L-arginine/ADMA than chronic patients. Further studies are needed to understand the functional significance of displayed DDAH2 polymorphisms in healthy and CVD individuals.