Poster Presenter

The Effects Of Silver Nanoparticles And Doxorubicin Complexes On The Structure of DNA and Inducing Of Apoptosis in T47D Cell Line
Azadeh Hekmat, Ali Akbar Saboury and Adeleh Divsalar
Iran

Breast cancer, which affects an important percentage of human beings, occurs when abnormal cells grow out of control in one or both breasts. Anthracyclines, particularly Doxorubicin are widely used antibiotics for medical treatments of breast cancer. In this research, we have studied the interaction between silver nanoparticles, DNA and anticancer drug of doxorubicin using UV-visible spectroscopy, fluorescence spectroscopy and the new solvation theory at 37°C. Our new solvation model was used to reproduce the thermodynamic parameters of the interaction between doxorubicin, DNA and silver nanoparticles. From this model, we have determined the binding constant and enthalpies of this interaction. The interaction of doxorubicin with varying silver nanoparticles concentration represented a set of two binding sites. Growth inhibitory and apoptosis effects of the doxorubicin and silver nanoparticles complexes toward the breast cancer cells (T47D) after 24 hours were measured. These results display that doxorubicin+silver nanoparticles have more growth inhibitory and inducing apoptosis against T47D, than doxorubicin alone. The result obtaining from this present study probably provide useful information to design better drug for breast cancer with less side effects and therefore developing more efficiency anti cancer drug in the future.