Poster Presenter

An Investigation of Ascorbic Acid Solid Lipid Nanoparticle Interaction on 3T3 Cell Line
G. Güney, H.M. Kutlu, L. Genç and A. Isca
Turkey

Ascorbic acid is toxic to tumour cells, and has been suggested as a anticancer agents. Ascorbic acid has several important functions in our body. In particular, ascorbic acid is a well known anti-oxidant that scavengers free radicals cells by hydrogen peroxide.

Solid lipid Nanoparticles (SLNs) are at the forefront of the rapidly developping field of nanotechnology with several potential applications in drug delivery, clinical medicine and research. SLN offer unique properties such as small size, large surface area, high drug loading and the interaction of phases at the interfaces. SLNs were prepared by homogenization method. Compritol® was used as the lipid. For the characterization of the formulations obtained, particle size distribution, Zeta potential measurement, thermal behavior (DSC), active agent content and pH were investigated.

We postulated that, ascorbic acid solid lipid nanoparticle interaction with rat embryo fibroblast cells, 3T3. We examined the cytotoxic effects of SLN ascorbic acid, again on 3T3 cells and determined cellular and ultrastructural of 3T3 cell by using MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and TEM.