Poster Presenter

Cardioprotective Effect Of Sesame Oil In An In-Vitro Model Of Myocardial Ischemic Reperfusion Injury
T.S. Mohamed Saleem and K. Gauthaman
India

The present study was designed to investigate the effects of oral administration of the sesame oil on in-vitro model of myocardial ischemic injury. The sesame oil was administered orally to Wistar albino rats (150–200 g) in three different doses (n=10), by gastric gavage [5 ml/kg: (S1), 10 ml/kg: (S2) and 15 ml/kg: (S3)] 6 days/week for 4 weeks. At the end of this period, Basal levels myocardial of endogenous antioxidants [EA] (SOD, catalase, reduced glutathione) and thiobarbituric acid reactive substances (TBARS) were estimated in control (C) and treated hearts. Control and sesame oil treated rat hearts were subjected to in-vitro global ischemic reperfusion injury (IRI) in Langendorff apparatus (ADInstruments, Australia), [initial 5 min equilibration period was followed by 9 min. of zero-flow (ischemia) and 12 min of re-flow, (reperfusion)]. A significant rise in myocardial thiobarbituric acid reactive substances (TBARS) and loss of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (suggestive of increased oxidative stress) occurred in the hearts subjected to in-vitro myocardial ischemic reperfusion injury. In sesame oil treated rats there was a significant decrease in TBARS and significant increase in endogenous antioxidants in all the groups. In 10 ml/kg treatment group, a significant rise in the levels of GSH, SOD and catalase were observed, and it shows better recovery profile than the other groups subjected to in-vitro ischemic reperfusion injury. In histological studies, all the groups, showed preserved myocardium. The present study demonstrates that the 10 ml/kg sesame oil augments endogenous antioxidant compounds of the rat heart and also prevents the myocardium from in-vitro model of myocardial ischemic reperfusion injury.