Poster Presenter

Evaluation Of Relation Between IL-4 and IFN-g Polymorphisms And Type 2 Diabetes
Ali Shamsizadeh and Mohammad Kazemi Arababadi
Iran

Background: Although, type 2 diabetes is the most frequent type of diabetes, the main cause of it yet to be clarified. Several environmental and genetic parameters are believed to be involved in diabetes. It has also been established that cytokines play key roles in pathogenesis of diabetes. Expression of cytokines is different from person to person and in different societies. Studies showed that polymorphisms of +874 of IFN-g and -590 of IL-4 are associated with the regulation of expression of these genes. In this study we aimed to find polymorphisms of these regions in type 2 diabetes patients.

Material and Methods: in this experimental study peripheral blood samples were collected from 51 type 2 diabetic patients and 50 healthy controls. DNA was extracted by salting out method and using ARMS-PCR and RFLP-PCR polymorphisms of +874 of IFN-g and -590 of IL-4, were analyzed.

Results: our findings indicated that TT genotype of IFN-g was increased in type 2 diabetic patients in compare to control but difference was not significant. Our results also have not shown any significant difference between IL-4 genotype in diabetic and healthy controls.

Discussion: our results suggested that TT genotype of IFN-g can be associated with diabetes. This association can be described by the fact that over expression of IFN-g shifted immune system to Th1, therefore, pancreatic cells were miscarried by immune cells.