Session Speaker

Anti-Angiogenic Effect of Silver Nanoparticles: Emergence of Targeted Therapeutic Molecule
Sangiliyandi Gurunathan

Angiogenesis is an important phenomenon involved in normal growth and wound healing processes. An imbalance of the growth factors involved in this process, however, causes the acceleration of several diseases including malignant, ocular, and inflammatory diseases. Inhibiting angiogenesis through interfering in its pathway is a promising methodology to hinder the progression of these diseases. Nanotechnology is the most promising field for generating new applications, however, only few nanoproducts are currently in use for medical purposes. The function and mechanism of silver nanoparticles (Ag-NPs) in angiogenesis have not been elucidated to date. PEDF is suggested to be a potent anti-angiogenic agent. In this study, we postulated that Ag-NPs might have the ability to inhibit angiogenesis, the pivotal step in tumor growth, invasiveness, and metastasis. We showed that Ag-NPs could also inhibit vascular endothelial growth factor (VEGF) induced cell proliferation, migration, and capillary-like tube formation of bovine retinal endothelial cells like PEDF. In addition, Ag-NPs effectively inhibited the formation of new blood microvessels induced by VEGF in the mouse Matrigel plug assay. To understand the underlying mechanism of Ag-NPs on the inhibitory effect of angiogenesis, we showed that Ag-NPs could inhibit the activation of PI3K/Akt. Together, our results indicate that Ag-NPs can act as an anti-angiogenic molecule by targeting the activation of PI3K/Akt signaling pathways. These findings are the first to reveal the novel functions of Ag-NPs in retinal angiogenesis and its molecular basis for the anti-angiogenic action.

Injected HM or IMMM with FITC labelled dendrímers (FITC-L-D) permitted confocal microscopy analysis of different sectioned tissues. Both liver and tumour sections showed cytoplasmic signal with perinuclear distribution. Not signal was detected at nuclear level The same perinuclear distribution pattern was observed when HPCMC were incubated with the FITC-L-D.

The results suggest that PAMAM-G4-dendrimers could be a promising compound in oncology diagnostic.