Session Speaker

A Combined Artificial Chromosome-Stem Cell Therapy Method for the Treatment of Krabbe's Disease in the Twitcher Mouse
Robert Katona, Ildiko Sinko, Tunde Praznovszky, Erika Csonka, Katalin Fodor, Imre Cserpan, Peter Blazso, Andor Udvardy and Gyula Hadlaczky
Hungary

Mammalian Artificial Chromosomes (MACs) are safe, stable, non-integrating genetic vectors with almost unlimited therapeutic transgene carrying capacity. The combination of MAC and stem cell technologies offers a new strategy for stem cell based therapy, the efficacy of which was confirmed and validated by using a mouse model of a devastating monogenic disease, galactocerebrosidase deficiency (Krabbe's disease).

Therapeutic MACs were generated by sequence-specific loading of galactocerebrosidase transgenes into a platform MAC, and stable, pluripotent mouse embryonic stem cell lines were established with these chromosomes. The transgenic stem cells were thoroughly characterized and used to produce chimeric mice on the mutant genetic background (Twitcher mice).

The lifespan of these chimeras was increased up to fourfold compared to control mice, verifying the feasibility of the development of MAC-stem cell systems for the delivery of therapeutic genes in stem cells to treat genetic diseases and cancers, and to produce cell types for cell replacement therapies.