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Effect of High Dose Thiamine on the Levels of Protein Biomarkers in Diabetes Mellitus Type 2
Samreen Riaz, Saadia Shahzad Alam, Naila Rabbani, Paul J Thornalley and M. Waheed Akhtar
Pakistan

This report describes the levels of protein biomarkers specific to diabetes mellitus type 2 and effect of high dose thiamine on these levels. Type 2 diabetic patients, age and sex-matched normal healthy controls were recruited from Sheikh Zayed Hospital, Lahore, Pakistan. Plasma proteins were analysed by 2-D liquid chromatographic system in which samples were initially fractionated by chromatofocusing and the selected fractions were further analysed by reverse-phase chromatography. The proteins which showed variation between test and control samples were identified by MALDI TOF analysis. Analysis of all the samples belonging to the control, placebo and thiamine treated groups were then analyzed for the four proteins which were found to vary, by ELISA. Levels of apolipoprotein A-I was found to decrease by -6.4 % while apolipoproetin-E, leptin and C reactive protein (CRP) were found to increase by +802, +218 and +872 %, respectively in the diabetic patients as compared to the controls. The level of CRP decreased by 63% after thiamine therapy as compared to the controls and the placebo while other protein markers did not show a significant change after the therapy. Since CRP level variation has been reported in other pathological sates, role of thiamine may have a significant bearing on the prognosis of such diseases.