Session Speaker

A New Strategy for the Synthesis of Nucleosides: One-Pot Enzymatic Transformation of Pentoses into Nucleosides
Igor A. Mikhailopulo Irina D. Konstantinova, Ilja V. Fateev, Roman S. Esipov, and Anatoly I. Miroshnikov
Belarus

We have recently suggested a new strategy for the synthesis of nucleosides (Bioorg. Med. Chem. 2006, 14, 6327). In the present communication, preliminary results of one-pot enzymatic transformation of D-ribose, 2-deoxy-D-ribose, or D-arabinose into the corresponding pyrimidine and purine nucleosides employing recombinant pure ribokinase (RK), phosphopentomutase (PPM) and nucleoside phosphorylases (NP), and the respective pyrimidine or purine heterobases will be reported. Search for the transformation conditions to achieve an optimal concerted action of RK [pentose → pentose-5-phosphate (P-5P)], PPM [P-5P → pentofuranose-1-a-phosphate (PF-1aP)], and NP (PF-1aP + heterobase → nucleoside) gave rise to the formation of pyrimidine or purine nucleosides in satisfactory yields.