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Sonodynamically induced antitumor effect of hematoporphyrin on breast adenocarcinoma
Dr. Mahboobeh Alamolhoda, M. Mokhtari-Dizaji, A. H. Barati, H. Hassanzade

Background: In sonodynamic therapy, a sonosensitizing agent can maintain a long time in tumor tissues and will produce cytotoxic agents such as free radicals after sonication. In this study, cytotoxic effects of hematoporphyrin (Hp) under ultrasound irradiation were investigated in murine model of breast adenocarcinoma in Balb/c mice.

Methods: The experiments were related to sonication by 1MHz ultrasound field with ISATA=2W/cm2 and 80% pulse mode. The spontaneous murine model of breast adenocarcinoma tumor was transplanted in the inbred Balb/c mice. For confidence from tumor type and their grading, tumor tissues were studied histophatologically. Fifty adult female Balb/C mice were used in this study. The tumor bearing mice with approximately 8-10mm diameters were divided into five groups: control group (A) sham group (B) ultrasound group (C) hematoporphyrin group (D) ultrasound and hematoporphyrin drug group (E). The volume and calculated tumor growth delay parameters of tumors were daily extracted for the 25-30 days, tertian.

Results: The tumor growth and relative volume of tumors in treated groups (C, D and E) are shown an anti-tumor effect in C and E groups. Ultrasound waves alone showed a slight antitumor effect which became increasingly significant as the hematoporphyrin was added with ultrasound (p<0.05). Conclusion: It is concluded that hematoporphyrin drug significantly sensitizes solid tumors to ultrasound demonstrating a synergistic antitumor effect.