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Production of a Novel Mineral-Based Hemostaic Agent for Controlling Life Threatening Arterial Bleeding
Mortazavi SMJ, Atef M , Roshan-shomal P, Raadpey N, Motazedi S and Darvish M

Introduction and Objective: Over the past decade the US army has widely studied new technologies for stopping sever hemorrhages and has introduced an effective Zeolite based hemostatic agent. On the other hand, Mortazavi and his colleagues recently reported the bio-stimulatory effects of the topical application of radioactive lantern mantle powder on wound healing (Mortazavi et al. 2009). Their subsequent studies showed significant changes in some histological parameters concerning healing. In this light, here the bio-stimulatory effect of burned radioactive lantern mantles powder as well as two minerals; bentonite and zeolite are presented.

Materials and Methods: This experimental study was conducted in the center for radiological studies, Shiraz University of Medical Sciences in 2008. Fifty male Wistar rats were divided randomly into 5 groups of 10 animals each. Following anesthesia, animals’ tails were cut at a thickness of 5 mm by using a surgical scissor. No intervention was made on the animals of the 1^st group. The 2^nd to 4^th group received topical non-radioactive lantern mantle powder, radioactive lantern mantle powder, Bentonite mineral or a mixture of Bentonite-Zeolite minerals respectively. After treatment with above mentioned agents, the volume of blood loss was measured using a scaled test-tube. The bleeding time and clotting time were also measured using a chronometer. SPSS software was used for statistical analysis. ANOVA was used for comparing the means of each parameter in the 5 groups.

Results: The volume of blood loss, bleeding and clotting times in control animals were 4.39±1.92 cc, 112.10±39.60 sec and 94.9±54.26 sec, respectively. In the 5^th group in which the animals were treated with a mixture of Bentonite-Zeolite minerals, the volume of blood loss, bleeding and clotting times were 1.31±0.60 cc, 34.50±4.65 sec and 24.2±4.61 sec, respectively.

Conclusion: This is the 1^st investigation in the world that studied the alterations of bleeding and clotting times following the use of lantern mantle powder as well as bentonite or the mixture of bentonite-zeolite minerals. The results obtained in this study clearly show the significant effect of the volume of blood loss as well as the bleeding time following the topical use of the mixture of bentonite-zeolite minerals. The radioactive and non-radioactive lantern mantle and the bentonite mineral alone had considerable effects but generally the magnitude of the effect was less than that of the mixture of bentonite-zeolite minerals.

Reference:
Mortazavi SMJ, Rahmani MR, Rahnama A, Saeed-Pour A, Nouri E, Hosseini N, and Aghaiee MM . The stimulatory effects of topical application of radioactive lantern mantle powder on wound healing. Dose Response. 2009; 7: 149-159.