PRELIMINARY SCREENING OF SOME PLANTS EXTRACTS USED IN TRADITIONAL MALARIA THERAPY IN KANO-NIGERIAN FOR ANTI-PLASMODIAL ACTIVITY
N.T. Dabo, M. Ofori, A.K. Nyarko, D. Edo and L. Bimi
Department of Microbiology, Bayero University, Kano, Nigeria.
Nine medicinal plants, distributed into eight families, used for treatment of malaria fever by the Hausa people of Kano-Nigeria were selected based on the traditional claims of the folks. These were collected and extracted using aqueous and organic solvents. A total of nineteen extracts and forty four extract-fractions were obtained.
Thirty extracts (7 aqueous extracts, 12 ethanol extracts and 11 methanol extracts-fraction) were selected and screened for their invitro anti-plasmodial activities against laboratory adapted chloroquine susceptible Plasmodium falciparum strain 3D7. Seven (23.33%) of these extracts that exhibited 70% or more inhibition at 50µg/mL concentration were further evaluated to establish their potency levels. The respective IC50 values obtained ranged between 0.361±0.15µg/ml and 37.67±4.20µg/ml. CALF1 from the leaves of C. aurantifolia (Rutaceae) and CBLF1 (a combination of the leaves of C. aurantifolia, Caricapapaya, Mangiferaindica and Psidiumguajava) were further screened against a clinical isolate of P. falciparum and recorded IC50 values similar to those obtained earlier against the laboratory strain, 12.42±3.94 µg/ml for CBLF1, and 29.69±0.65µg/ml for CALF1 respectively. It could be concluded that majority of the plants have demonstrated appreciable concentration dependant invitro anti-plasmodial effects against the laboratory adapted and field strains P. falciparum.
Keywords: Plants Extracts, Traditional Malaria Therapy, Hausa people, Kano and Nigeria.