CARDIAC-LIKE TRANS-DIFFERENTIATION OF HUMAN MEDIASTINAL ADIPOSE DERIVED MESENCHYMAL STEM CELLS AFTER CULTURE IN CARDIOSPHERES CONDITIONED MEDIA
Camilla Siciliano, Chiara Napoletano, Isotta Chimenti, Mohsen Ibrahim, Antonella Calogero, Erino Angelo Rendina, Giorgio Mangino, Gaia Scafetta, Martina Leopizzi, Mariangela Peruzzi, Giacomo Frati and Elena De Falco
University of Rome Sapienza, Faculty of Pharmacy and Medicine, Department of Medical-Surgical Science and Biotechnologies, C.so della Repubblica 79 04100 Latina, Italy.
Nowadays, cardiac regenerative medicine is still facing some limitations because of the complexity to find the most suitable stem cell source and due the low efficiency in trans-differentiation yield achieved in vitro. Recently, human Adipose-derived MSCs (hADMSCs) have become an attractive cell source because they have shown to reduce the infarcted area in vivo and that cardiac differentiation of hADMSCs could be achieved spontaneously using 5-Azacytidine or exposing them to extract of rat cardiomyocytes. In 2012 a multi-potent stem cell population from mediastinal depots has been characterized, providing the evidence of a stem cell population in this site of the body. In this study we investigated the potential of human mediastinal Adipose-derived Mesenchymal stem cells (hmADMSCs) to trans-differentiate into a cardiac-like lineage after preconditioning with cardiospheres conditioned media (CCM). Cardiospheres (CSs) are a widely characterized in vitro 3D model of spontaneous niche-like microtissue, housing cardiac progenitor cells and supporting mesenchymal cells. Results indicated that CCM affected cell morphology but not proliferation (p>0.05). RT-PCR data indicated that expression levels of multiple cardiogenic genes (KDR, Nkx2.5, GATA-4, MHC, SMA, c-Kit, Thy-1, TnI, Cx43) are modulated similarly to Cardiospheres (CSs) and comparably to the treatment with 5-Azacitidine. For the first time, we demonstrated that hmADMSCs holds a plastic stem cells pool able to trans-differentiate invitro into cardiac-like lineage.