SOX2 REPROGRAMS FIBROBLASTS IN TO THE INDUCED NEURAL STEM CELLS
Celther Polska Sp. z o. o. Milionowa street 23; 90-364 Łódź, Poland
Several laboratories conduct research to develop not only iPSc but also induced multipotent stem cells. Those cells seems to be less dangerous for future treatment since cannot be source of teratomas. We have observed different types of reprogrammed cells, after using Life Technologies Sendai virus - Cyto Tune system. It is possible since four independent and separated viral vectors are present in this system. One type of cells – showing only SOX2 expression was taken in to more systematic analyses. Obtained cells showed features of GFAP positive Neural Stem Cells. Those induced Neural Stem cells (iNS) could be differentiated in to the glial and neuronal cells. iNS cells did not cause presence of teratomas in SCID mice. Our works supports Cell-Stem Cell, K.L Ring etal. publication.
This study was supported by Polish Agency for Enterprise Development POIG.01.04.00-10-011/11