Cardiovascular Drug Discovery & Therapy (Track)


Intskirveli N., BasiShvili E., Sanikidze T. and Sharashenidze G.

Department of Physics and Biophysics, Department of Endocrinology, Tbilisi State medical University, 33 Vaja Pshavela, Tbilisi 0177, Georgia


The common main sign of the most risk-factors of atherosclerosis is intensification of oxidative processes, intensified production of reactive compound of oxygen and nitrogen. The development of disbalance of body redox-status leads to LDL oxidation (oxLDL), which is essential for the development of atherosclerotic plaques in blood vessel walls. It is supposed that infiltration of T-lymphocytes activated oxLDL in atherosclerotic plaques at early stage of atherogenesis, contributes to enhancement of apoptosis of T-cells. Reduced clearance of the apoptotic products in enriched environment with oxidants, hyperproduction of pro-inflammatory cytokines and oxidants (reactive compounds of oxygen and nitrogen) on vascular smooth muscle cells, have cytotoxic effect and contribute to deepening of atherogensis. The goal of this work is to explore the antiatherogenic action of the peel extract of Georgian Red Grape on Jurkat cells in oxidative environment of these cells (with hydrogen peroxide) in conditions of incubation. Jurkat type human lymphoblastic T-cells are well-established model to search apoptotic mechanisms and to analyze inductive and depressing influence of the products on apoptosis.

Conclusion: By EPR-spectroscopy we determined, that the peel extract of Georgian Red Grape contributes to the reduction in hyperproduction of reactive oxygen, lypoperoxides and NO, normalization of the activity of antioxidative enzymes (catalase, superoxide dismutase and glutathione reductase), regeneration of energogenesis in mitochondrions, reduction in intensity of apoptosis and normalization of cell proteins.