EDUCATION/TRAINING(Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and residency training if applicable.)

Personal Statement

My scientific interests focus on chemically exploring and biosynthetically exploiting marine bacterial natural products as drug leads. Our research laboratory has contributed to the rational chemoenzymatic synthesis and genetic engineering of designer molecules through the discovery and detailed characterization of novel biosynthetic enzymes and their encoding genes. We further employ a genomics-guided discovery approach that has resulted in the rational discovery of new chemical entities. The Moore group at UCSD-SIO presently consists of 6 graduate students, 10 postdocs, a project scientist and several visiting scientists from chemistry, biochemistry, microbiology and molecular biology backgrounds to create a stimulating and diverse work group able to successfully spearhead multidisciplinary research projects.

Positions and Honors

Positions and Employment

Selected Peer-reviewed Publications (selected from >140 peer-reviewed publications)

1.DW Udwary, L Zeigler, R Asolkar, V Singan, A Lapidus, W Fenical, PR Jensen, BS Moore. Genome sequencing reveals complex secondary metabolome in the marine actinomycete Salinispora tropica. Proc Natl Acad Sci USA, 104, 10376-10381 (2007). PMCID: PMC1965521

2.Q Cheng, L Xiang, M Izumikawa, D Meluzzi, BS Moore. Enzymatic total synthesis of enterocin polyketides. Nature Chem Biol, 3, 557-558 (2007). PMID: PMC17704772

3.AS Eustáquio, F Pojer, JP Noel, BS Moore. Discovery and characterization of a marine bacterial SAM- dependent chlorinase. Nature Chem Biol, 4, 69-74 (2008). PMCID: PMC2762381

4.AS Eustáquio, RP McGlinchey, Y Liu, C Hazzard, LL Beer, G Florova, MM Alhamadsheh, A Lechner, AJ Kale, Y Kobayashi, KA Reynolds, BS Moore. Biosynthesis of the salinosporamide A polyketide synthase substrate chloroethylmalonyl-CoA from S-adenosyl-L-methionine. Proc Natl Acad Sci USA, 106, 12295- 12300 (2009). PMCID: PMC2718359

5.Y Liu, C Hazzard, AS Eustáquio, KA Reynolds, BS Moore. Biosynthesis of salinosporamides from ,β - unsaturated fatty acids: Implications for extending polyketide synthase diversity. J Am Chem Soc, 131, 10376-10377 (2009). PMCID: PMC2737082

6.MC Wilson, TAM Gulder, T Mahmud, BS Moore. Shared biosynthesis of the saliniketals and rifamycins in Salinispora arenicola is controlled by the sare1259-encoded cytochrome P450. J Am Chem Soc, 132, 12757-12765 (2010). PMCID: PMC2946249

7.P Bernhardt, T Okino, JM Winter, A Miyanaga, BS Moore. A stereoselective vanadium-dependent chloroperoxidase in bacterial antibiotic biosynthesis. J Am Chem Soc, 133, 4268-4270 (2011). PMCID: PMC3065929

8.A Miyanaga, JE Janso, L McDonald, M He, H Liu, L Barbieri, AS Eustáquio, EN Fielding, GT Carter, PR Jensen, X Feng, M Leighton, FE Koehn, and BS Moore. Discovery and assembly line biosynthesis of the lymphostin pyrroloquinoline alkaloid family of mTOR inhibitors in Salinispora bacteria. J Am Chem Soc, 133, 13311-13313 (2011). PMCID: PMC3161154

9.RD Kersten, Y-L Yang, Y Xu, P Cimermancic, S-J Nam, W Fenical, MA Fischbach, BS Moore, and PC Dorrestein. A mass spectrometry-guided genome mining approach for natural product peptidogenomics, Nature Chem Biol, 7, 794-802 (2011). PMCID: PMC3258187

10.Y Xu, RD Kersten, S-J Nam, L Lu, AM Al-Suwailem, H Zheng, W Fenical, PC Dorrestein, BS Moore, and P-Y Qian. Bacterial biosynthesis and maturation of the didemnin anticancer agents, J Am Chem Soc, 134, 8625- 8632 (2012). PMCID: PMC3401512

11.K Yamanaka, KS Ryan, TAM Gulder, CC Hughes, and BS Moore. Flavoenzyme-catalyzed atroposelective N,C-bipyrrole homocoupling in marinopyrrole biosynthesis, J Am Chem Soc, 134, 12434-12437 (2012). PMCID: PMC3415713

12.AC Ross, Y Xu, L Lu, RD Kersten, Z Shao, AM Al-Suwailem, PC Dorrestein, P-Y Qian, BS Moore. Biosynthetic multitasking facilitates thalassospiramide structural diversity in marine bacteria, J Am Chem Soc, 135, 1155-1162 (2013). PMCID: PMC3563429

13.A Lechner, MC Wilson, YH Ban, J-y Hwang, YJ Yoon, BS Moore. Designed biosynthesis of 36-methyl- FK506 by polyketide precursor pathway engineering, ACS Syn Biol, 2, 379-383 (2013). PMCID: PMC3716868

14.R Teufel, A Miyanaga, Q Michaudel, F Stull, G Louie, JP Noel, PS Baran, B Palfey, BS Moore. Flavin- mediated dual oxidation controls an enzymatic Favorskii-type rearrangement. Nature, 503, 552-556 (2013). PMCID: PMC3844076

15.RD Kersten, N Ziemert, D Gonzales, BM Duggan, V Nizet, PC Dorrestein, BS Moore. Glycogenomics as a mass spectrometry-guided genome mining method for microbial glycosylated molecules. Proc Natl Acad Sci USA, 110, E4407-E4416 (2013). PMCID pending

Research Support

The major goals of this project are to study the salinosporamide biosynthetic enzymology, chemoenzymatically prepare new derivatives for biological evaluation, and to further explore the evolved proteasome resistance mechanism in the salinosporamide-producing bacterium to contrast against emerging human proteasome resistance to this growing class of drugs.

The major goals of this project are to study the biosynthetic enzymology of the enterocin, napyradiomycin, marinopyrrole and chlorizidine marine antibiotics and to explore new enzymatic reactions by high-resolution protein crystallography.

NIH/NIGMS R01 GM97509 Moore (PI) 4/1/12–2/29/16 “Experiment Based Genome Mining of Ribosomal Natural Products”.

The major goals of this project are to further develop and apply a mass spectrometry-guided genome mining platform to discover and characterize microbial natural product ribosomal and nonribosomal peptide natural products and their biosynthetic machinery.

NIH/NIGMS R01 GM857770 Moore (PI) 8/1/09–7/31/17 “Natural Product Genome Mining”.

The major goal of this project is to explore and exploit the natural product biosynthetic capabilities of marine bacteria belonging to the genus Salinispora using a genomics approach.

The goal of the Scripps OHH Center is to identify, quantify and determine the biological source(s) of small, natural, brominated organic compounds that bioaccumulate and are available to enter the human population through sea food consumption. This multi-investigator Center aims to provide a complete view of brominated natural product cycling in coastal California with the added benefit of identifying the actual source and biosynthetic mechanism of production. This project is co-sponsored by the NSF.

NSF OCE-1313747 Moore (PD) 3/1/13–2/28/18 “Scripps Center for Oceans and Human Health”.

NSF is the co-sponsor of the NIH/NIEHS grant P01 ES021921.