Regenerative Medicine   (Track)

CULTURE OF MESENCHYMAL STEM CELLS ON GRAPHEN-COLLAGEN SCOFFOLD

Fatemeh Kermanian

Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

Abstract

Aim: Neural stem cell (NSC) based therapy provides a promising approach for neural regeneration. For the success of NSC clinical application, a scaffold is required to provide three-dimensional (3D) cell growth microenvironments and appropriate synergistic cell guidance cues. Recently, the use of scaffolds has been considered in tissue engineering. The purpose of this study was to prepare a conductive scaffold and investigate the viability of seeded rat bone marrow stem cells on that.

In this experimental study, synthesis of graphene and collagen-graphene films using chemical vapor deposition. Then rat bone marrow mesenchymal stem cells were seeded on the scaffold.

The Viability of cells was evaluated using MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide) test on days 1, 5, 10, 15 and 20. The number of viable cells on the scaffold showed a significant increase (P< 0.05) 20 days after seeding compared to day 1 and 5.

It was found that graphene - collagen scaffolds can not only support NSC growth, but also keep cell at an active proliferation state. Our findings implicate graphene - collagen scaffolds could offer a powerful platform for NSC research, neural tissue engineering and neural prostheses.

Keywords: Scaffold, graphen, stem cell.