Innovative Drug Discovery and Nanotechnology  (Track)

TARGETED MAGNETIC HYPERTHERMIA BY DENDRITIC CELLS MORPHOGENIC PROTEIN1

Gerardo F. Goya and M.Ricardo Ibarra

Nanoscience Institute of Aragon (INA), University of Zaragoza, Pedro Cerbuna 12, 50009- Zaragoza, Spain

Abstract

We aim to investigate the effects of alternating magnetic fields (AMF) on the death rate of magnetic nanoparticles uploaded dendritic cells (DCs). Specific parameters as the time of application; the magnetic field amplitude and the concentration of magnetic nanoparticles (MNP) used as heating agents have been investigated in order to assess the best conditions for a controlled field-induced cell death.

The magnetic hyperthermia experiments were carried on monocyte-derived DCs at f = 260 kHz and field amplitudes H up to 12.7 kA/m. Application times of 5, 10 and 15 min were tested for several concentration of co-incubated MNPs (from 1 to 50 µg MNP/ml medium). Cell viability was measured by Trypan blue, MTT and flow cytometry using annexin-propidium iodide markers.

We were able to control the DCs viability by choosing a) field amplitude; b) application time; and c) the concentration of co-incubated MNPs. Large decrease of cell viability induced by AMF was observed without heating of the cell culture [1]. In such a case AMF-triggered mechanisms could be the origin of the observed cell death, yielding a different cell death pathway.

We propose that the cell death of magnetically loaded DCs constitutes an appealing strategy to be used as a non-invasive and selective hyperthermia method. This can be controlled by adequate tuning of the physical AMF parameters and/or MNPs concentration. Power absorption by the MNPs might locally trigger metabolic processes yielding fast and irreversible cell damage [2]. We have addressed the effects of radio frequency fields and determined the strong effect on MNP loaded eukaryotic cells [3].

Keywords: Magnetic nanoparticles, deep cancer treatment, magnetic hyperthermia.

REFERENCES

[1] I Marcos-Campos, L Asin, T E Torres, C Marquina, A Tres, M R Ibarra and G F Goya, Nanotechnology 22 (2011) 205101,doi.org/10.1088/0957-4484/22/20/205101
[2] L. Asín, G.F. Goya, A. Tres and M.R. Ibarra, Cell Death and Disease (2013) 4, e596; doi:10.1038/cddis.2013.121
[3] G.F. Goya, L. Asín and M.R. Ibarra, Int J Hyperthermia, (2013) 29(8) 810-8. doi: 10.3109/02656736.2013.83864